P-290 Choosing the appropriate fertilization method would increase fertilization rate and improve embryo quality

Abstract Study question The impact of Conventional IVF versus ICSI on embryo morphokinetic is still scarce. Would proper selection fertilization method affect embryonic development in different subgroups? Summary answer In unexplained or tubal factor subgruops, convential was preferable resulting higher rate and better quality expressed by less discarded embryos. What known already requires a sperm cell to penetrate the cumulus cells zona pellucida, but can induce insemination without certain processes taking place. Moreover, more invasive than conventional IVF. For example, oocytes are exposed hyaluronidase intense light during denuding process damaged mechanical pipetting. It found that ICSI-fertilized 4-cell embryos spent approximately 2.5 hours time 2-cell stage IVF-fertilized embryos, 3-cell longer oocytes. addition, first cleavage has been reported be slow design, size, duration A retrospective analysis prospective collective data all fresh ART (assisted reproductive technique) cycles, conducted underwent single clinic center. Participants/materials, setting, methods study collected total 11472 fertilized were studied using time-lapse system (Embryoscope). 2085 (conventional group) 9387 (ICSI group). All sibling investigated for developmental events. Main results role chance Significantly Younger maternal age (34.6 vs. 34.1; P < 0.001) male infertility (43% 14%; group. Normal (2PN) significantly (98% 89.9%). transferred (37% vs 33%; 0.001( group frozen 59% 46.5%; 0.001). parameters morphokinetics starting pronuclear appearance till morula shorter. KID score comparable between groups (3.23 3.3 = 0.08), however, (16.5% 8% p Limitations, reasons caution This study. We can’t report our cumulative pregnancy since not yet. Wider implications findings Regardless factor, given debate techniques daily spotlight anovulation, enable us offer personalized treatment. Trial registration number 026-20-HWMC